# 1355: HYPHOMICROBIUM MEDIUM ## Main sol. 1355 2.5 g K2HPO4 2.25 g NaH2PO4 0.5 g (NH4)2SO4 0.2 g MgSO4 x 7 H2O 1 ml **Visniac Trace Elements (1000x)**, pH 6 1000 ml Distilled water 12 g Agar, for solid medium 2 ml Methylamine (0.2% v/v) 1. Adjust to pH 6 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar. After autoclaving add sterile methylamine (0.2% v/v) to the sterile main solution. ## Visniac Trace Elements (1000x) 5 g EDTA 2.2 g ZnSO4 x 7 H2O 0.733 g CaCl2 x 2 H2O 0.506 g MnCl2 x 4 H2O 0.499 g FeSO4 x 7 H2O 0.11 g (NH4)6Mo7O24 x 4 H2O 0.157 g CuSO4 x 5 H2O 0.161 g CoCl2 x 6 H2O 500 ml Distilled water 1. Adjust pH to 6.0 with NaOH.