# 1356: HMD MEDIUM


## Mineral Salts Solution

0.1	g	Yeast extract	
0.5	g	Casamino acids	
0.1	g	CaCl2 x 2 H2O	
20	g	MgCl2 x 6 H2O	
5	g	K2SO4	
0.5	g	NH4Cl	
0.05	g	KH2PO4	
180	g	NaCl	
1000	ml	Distilled water	
12	g	Agar, for solid medium	

1. Adjust to pH 7.5 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar.