# 1359: PHAEOVIBRIO SULPHIDIPHILUS MEDIUM


## Main sol. 1359

0.5	g	KH2PO4	
0.5	g	MgSO4 x 7 H2O	
0.4	g	NaCl	
0.68	g	NH4Cl	
0.25	g	CaCl2 x 2 H2O	
0.5	g	Na pyruvate	
0.5	g	Sodium succinate	
0.5	g	Na acetate	
0.5	g	Yeast extract	
5	ml	Ferric citrate (0.1%)	
15	ml	NaHCO3 (10%)	
1	ml	Na2S x 9 H2O (1M)	
1	ml	Vitamin B12 (2mg/100ml)	
1	ml	**Trace element solution SL7**	
1000	ml	Distilled water	

1. Prepare the basal medium under nitrogen.
2. NaHCO3 solution (autoclaved in sealed, half full vessels under CO2), Na2S x 9H2O solution (autoclaved) and vitamin B12 solution (filter sterilised) is added to the autoclaved medium and the final pH adjusted to 6.6 - 7.4.


## Trace element solution SL7

1	ml	HCl (25%, v/v)	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
60	mg	H3BO3	
200	mg	CoCl2 x 6 H2O	
20	mg	CuCl2 x 2 H2O	
20	mg	NiCl2 x 6 H2O	
40	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water