# 1365a: 1/10 R2A MEDIUM MODIFIED (+ Yeast Extract) ## Mineral Salts Solution 0.25 g Yeast extract 0.05 g Proteose peptone (Difco no. 3) 0.05 g Casamino acids 0.05 g Glucose 0.05 g Starch (soluble) 5 mg MgSO4 x 7 H2O 15 g Agar 1000 ml Distilled water 1. Final pH 4.5 - 5.0; adjust with Alginic Acid before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.