# 137: MINERAL LACTATE MEDIUM (low carbon content)


## Main sol. 137

0.5	g	Na-lactate	
1.13	g	K2HPO4 x 3 H2O	
0.88	g	KH2PO4	
1	g	NH4Cl	
0.5	g	MgSO4 x 7 H2O	
1	g	NaCl	
5	mg	CaCl2 x 2 H2O	
1.2	ml	**Trace element solution (Vishniac and Santer, 1957)**	
1000	ml	Distilled water	

1. Adjust pH to 7.0.


## Trace element solution (Vishniac and Santer, 1957)

50	g	Na2-EDTA	
22	g	ZnSO4 x 7 H2O	
5.54	g	CaCl2 x 2 H2O	
5.06	g	MnCl2 x 4 H2O	
5	g	FeSO4 x 7 H2O	
1.1	g	(NH4)6Mo7O24 x 4 H2O	
1.57	g	CuSO4 x 5 H2O	
1.61	g	CoCl2 x 6 H2O	
1000	ml	Distilled water	

1. Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.