# 1370: RICH ORGANIC MEDIUM


## Main sol. 1370

0.3	g	MgSO4 x 7 H2O	
0.3	g	KH2PO4	
0.1	g	NH4Cl	
1	g	CaCl2 x 2 H2O	
1	g	Na-acetate	
0.5	g	Yeast extract	
0.5	g	Peptone	
0.5	g	Casamino acids	
1000	ml	Distilled water	

1. Adjust pH to 5.9. Add NaOH (0.1 mol/l) 16ml/l after sterilisation from sterile stock solution to readjust pH to 6.8.
2. Add the following from sterile stock solutions to the autoclaved medium:

2	ml	**Trace elements solution N1**	
2	ml	**Vitamin solution N1**	


## Vitamin solution N1

400	mg	Nicotinic acid	
400	mg	Thiamine	
80	mg	Biotin	
20	mg	Vitamin B12	
1000	ml	Distilled water	

1. Filter sterilised


## Trace elements solution N1

300	mg	FeSO4 x 7 H2O	
5	mg	ZnSO4 x 7 H2O	
3	mg	MnCl2 x 4 H2O	
2	mg	H3BO3	
5	mg	CoCl2 x 6 H2O	
1	mg	CuCl2 x 2 H2O	
2	mg	NiCl2 x 6 H2O	
3	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water