# 1374: IGNAVIBACTERIA MEDIUM


## Main sol. 1374

1	g	NaCl	
0.4	g	MgCl2 x 6 H2O	
0.5	g	KCl	
0.1	g	CaCl2 x 2 H2O	
0.25	g	NH4Cl	
0.2	g	KH2PO4	
4	g	Na2SO4	
1	ml	**Trace element solution**	
2	g	Yeast extract	
0.1	g	NaHCO3	
990	ml	Distilled water	

1. Autoclave at 121 °C for 15 min.
2. Add filter-sterilized vitamin solution (1ml/10ml)

10	ml	**Vitamin solution**	


## Trace element solution

784	mg	Fe(NH4)2(SO4)2 x 6 H2O	
143.6	mg	ZnSO4 x 7 H2O	
99	mg	MnCl2 x 4 H2O	
94.6	mg	Na2SeO4	
6	mg	H3BO3	
33	mg	Na2WO4 x 2 H2O	
1.8	mg	CuCl2 x 2 H2O	
238	mg	CoCl2 x 6 H2O	
395	mg	(NH4)2Ni(SO4)2 x 6 H2O	
24	mg	Na2MoO4 x 2 H2O	
10	ml	HCl (concentrated)	
990	ml	Distilled water	


## Vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine-HCl x 2 H2O	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	D-Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water