# 1377: HALOGRANUM MEDIUM


## Main sol. 1377

1	g	Yeast extract	
1	g	Proteose peptone no. 3	
1	g	Casamino acids	
1	g	Glucose	
0.05	g	Sodium pyruvate	
0.6	g	K2HPO4	
132	g	NaCl	
1000	ml	Sea water (natural )	
20	g	Agar, for solid medium	

1. Adjust to pH 7.0 and sterilize by autoclaving.
2. The medium may be solidified by adding 20.0 g/l agar. In this case, NaCl needs to be autoclaved separately.