# 1378: MO-CFXI MEDIUM


## Main sol. 1378

25	g	NaCl	
4	g	MgCl2 x 6 H2O	
0.5	g	KCl	
1	g	CaCl2 x 2 H2O	
0.535	g	NH4Cl	
0.1	g	KH2PO4	
1	g	Tryptone	
1.8	g	Glucose	
0.55	g	Sodium pyruvate	
1	g	Yeast extract	
2	g	NaHCO3	
0.3	g	Cysteine-HCl	
1	ml	Resazurin (1mg/ml)	
1	ml	**Trace element solution**	
1000	ml	Distilled water	
0.03	%	Na2S	
2	ml	**Vitamin solution**	

1. pH 7.0
2. Prepare under N2/CO2 (80/20) gas.
3. Autoclave and add the following components after autoclaving from stock solutions:
4. sterile Na2S solution end concentration 0.03%
5. and filter-sterile Vitamin solution (see below) 0.2 ml / 100 ml.


## Trace element solution

1.27	g	FeCl2 x 4 H2O	
0.14	g	ZnCl2	
0.2	g	MnCl2 x 4 H2O	
2	mg	Na2SeO4	
6	mg	H3BO3	
3	mg	Na2WO4 x 2 H2O	
1	mg	CuCl2 x 2 H2O	
0.13	g	CoCl2 x 6 H2O	
0.01	g	AlCl3	
0.01	g	NiCl2	
0.02	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## Vitamin solution

4.9	mg	Biotin	
8.8	mg	Folic acid	
4.1	mg	Pyridoxine hydrochloride	
6.7	mg	Thiamine-HCl x 2 H2O	
7.5	mg	Riboflavin	
2.4	mg	Nicotinic acid	
9.5	mg	D-Calcium pantothenate	
0.1	mg	Vitamin B12	
2.7	mg	p-Aminobenzoic acid	
4.1	mg	Lipoic acid	
1000	ml	Distilled water