# 1381: ½ S° HELIORESTIS MEDIUM


## Main sol. 1381

1	ml	EDTA (1%)	
0.5	g	KH2PO4	
0.5	g	NH4Cl	
1.63	g	Bicine	
0.2	g	MgSO4 x 7 H2O	
0.075	g	CaCl2	
2.5	g	Na2CO3	
2.5	g	NaHCO3	
1	g	Na-acetate	
0.1	g	Yeast extract	
0.25	g	Na2S x 9 H2O	
1	g	Na-pyruvate	
1	ml	**Trace elements**	
20	µg	Vitamin B12	
1000	ml	Distilled water	

1. Prepare under N2 gas and add vitamin B12, NaHCO3, Na2CO3 and sulfide as single sterile stock solutions to sterile medium after autoclaving. Adjust pH to 9 - 9.5.


## Trace elements

5.2	g	EDTA	
190	mg	CoCl2 x 6 H2O	
0.1	g	MnCl2 x 4 H2O	
1.5	g	FeCl2 x 4 H2O	
6	mg	H3BO3	
17	mg	CuCl2 x 2 H2O	
188	mg	Na2MoO4 x 2 H2O	
25	mg	NiCl2 x 6 H2O	
70	mg	ZnCl2	
30	mg	VOSO4 x 2 H2O	
2	mg	Na2WO4 x 2 H2O	
2	mg	NaHSeO3	
1000	ml	Deionized water