# 1382: MP MEDIUM


## Main sol. 1382

0.1	g	KH2PO4	
0.02	g	CaCl2 x 2 H2O	
0.2	g	NH4Cl	
0.1	g	Yeast extract	
0.5	g	Glucose	
1	ml	**Trace element solution (SLA)**	
1000	ml	Distilled water	

1. Adjust pH to 5.0-6.5
2. Serum flasks are filled with Medium by 5/6 of the total volume.
3. Sterilize at 121°C for 15 minutes.


## Trace element solution (SLA)

1.8	g	FeCl2 x 4 H2O	
250	mg	CoCl2 x 6 H2O	
10	mg	NiCl2 x 6 H2O	
10	mg	CuCl2 x 2 H2O	
70	mg	MnCl2 x 4 H2O	
100	mg	ZnCl2	
500	mg	H3BO3	
30	mg	Na2MoO4 x 2 H2O	
10	mg	Na2SeO3 x 5 H2O	
1000	ml	Distilled water	

1. Adjust pH to 2.0 - 3.0.