# 1383: VL55 MEDIUM modified


## Main sol. 1383

1.95	g	MES (2-Morpholinoethanesulfonic acid)	
10	g	MgSO4 x 7 H2O (20mM)	
10	g	CaCl2 x 2 H2O (30mM)	
10	g	(NH4)2HPO4 (20mM)	
1	ml	**Selenite-tungstate solution**	
1	ml	**Trace element solution SL-10**	
940	ml	Distilled water	
3	ml	**Vitamin solution**	
2	mM	Glucose	

1. Adjust to pH 5.5 with NaOH/KOH (about 6 ml).
2. Autoclave and add 3 ml/l vitamin solution (see Medium1266) and 2mM Glucose end-concentration.


## Selenite-tungstate solution

1000	ml	Distilled water	
0.5	g	NaOH	
3	mg	Na2SeO3 x 5 H2O	
4	mg	Na2WO4 x 2 H2O	


## Trace element solution SL-10

10	ml	HCl (25%; 7.7 M)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	


## Vitamin solution

17	mg	Vitamin B12	
13	mg	4-aminobenzoate	
3	mg	Biotin	
33	mg	Nicotinic acid	
17	mg	Hemicalcium D-(+)-pantothenate	
50	mg	Pyridoxamine hydrochloride	
33	mg	Thiamine-HCl x 2 H2O	
10	mg	DL-6,8-thioctic acid	
10	mg	Riboflavin	
4	mg	Folic acid	
1000	ml	Distilled water	

1. Filter sterilize the solution.