# 1390: RHODOVULUM MEDIUM


## Main sol. 1390

0.1	g	Yeast extract	
1	g	KH2PO4	
1	g	NH4Cl	
0.7	g	Na2SO4	
120	g	NaCl	
3	g	NaHCO3	
0.2	g	Na2S x 9 H2O	
0.5	g	MgCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
0.5	g	Na-ascorbate	
1	g	Na-malate	
1	ml	**Trace element solution (SLA)**	
20	µg	Vitamin B12	
1	ml	**Vitamin solution (VA)**	
20	µg	Vitamin B12	
1000	ml	Distilled water	

1. Adjust ph to 7.0
2. Prepare the medium under N2 gas without the NaHCO3, Na2S x 9 H2O, VA-Vitamin solution and vitamin B12. Autoclave and add the VA-Vitamin solution and vitamin B12 from a filter-sterilised stock solution and the NaHCO3, Na2S x 9 H2O, from sterile stock solutions. The final pH of the medium should be 7.0.


## Trace element solution (SLA)

1.8	g	FeCl2 x 4 H2O	
250	mg	CoCl2 x 6 H2O	
10	mg	NiCl2 x 6 H2O	
10	mg	CuCl2 x 2 H2O	
70	mg	MnCl2 x 4 H2O	
100	mg	ZnCl2	
500	mg	H3BO3	
30	mg	Na2MoO4 x 2 H2O	
10	mg	Na2SeO3 x 5 H2O	
1000	ml	Distilled water	

1. Adjust pH to 2.0 - 3.0.


## Vitamin solution (VA)

10	mg	Biotin	
35	mg	Nicotinic acid amide	
30	mg	Thiamine-HCl x 2 H2O	
20	mg	p-Aminobenzoic acid	
10	mg	Pyridoxal hydrochloride	
10	mg	Calcium pantothenate	
5	mg	Vitamin B12	
100	ml	Distilled water