# 1391: MMJHSY MEDIUM (modified)


## Main sol. 1391

20	g	NaCl	
0.09	g	K2HPO4	
0.07	g	KH2PO4	
0.8	g	CaCl2 x 2 H2O	
1	g	NH4Cl	
1	g	NaNO3	
4	g	MgSO4 x 7 H2O	
3	g	MgCl2 x 6 H2O	
0.33	g	KCl	
0.01	g	FeSO4 x 7 H2O	
2	g	Na2S2O3 x 5 H2O	
0.01	g	Fe(III) citrate	
2	g	Yeast extract	
5	ml	**Trace mineral solution**	
1000	ml	Distilled water	

1. Prepare the medium under N2/CO2 (80%N2, 20%CO2) and fill in , the pH of the medium is adjusted with NaOH to 6.8. After autoclaving, the separately autoclaved, concentrated solutions including each of follows are added to the medium. Then a mix gas (80%H2, 20%CO2) is purged for 5 min.
2. Finally, the mix gas (77%H2, 20%CO2, 3%O2) is compressed into gas phase (>80% volume of the tube or bottle) at 2 atm. 

1	ml	**Wolin's vitamin solution**	
0.1	%	NaHCO3	


## Trace mineral solution

1.5	g	Nitrilotriacetic acid	
0.5	g	MnSO4 x n H2O	
0.5	g	CoSO4 x 7 H2O	
0.18	g	ZnSO4 x 7 H2O	
0.01	g	CuSO4 x 5 H2O	
0.02	g	AlK(SO4)2 x 12 H2O	
0.01	g	H3BO3	
1	mg	Na2MoO4 x 2 H2O	
0.01	g	SrCl2 x 6 H2O	
0.01	g	NaBr	
0.01	g	KI	
0.1	g	NiCl2 x 6 H2O	
0.1	g	Na2SeO3 x 5 H2O	
1	g	Fe2(SO4)3 x n H2O	
0.1	g	H2WO4	
1000	ml	Distilled water	


## Wolin's vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium D-(+)-pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	(DL)-alpha-Lipoic acid	
1000	ml	Distilled water