# 1398: 1/10 PYGV MEDIUM modified


## Main sol. 1398

75	ml	**S I**	
75	ml	**S II**	
1	g	Trypticase	
0.5	mg	Yeast extract	
1	g	Glucose	
0.3	mg	L-Cysteine HCl	
0.5	ml	Resazurin (0.1%)	
10	ml	**100x Vitamin solution**	
1000	ml	Distilled water	

1. SII 
2. pH 7.0
3. Prepare the medium without the vitamin solution under an atmosphere of N2 and dispense into Hungate tubes. Seal under an atmosphere of nitrogen with rubber stoppers and autoclave. Aseptically add the vitamin solution from a filter sterilised stock solution to the sterile medium.


## 100x Vitamin solution

0.1	g	Biotin	
0.1	g	Cobalamine	
0.5	g	Thiamine-HCl x 2 H2O	
0.3	g	p-Aminobenzoic acid	
1.5	g	Pyridoxine	
0.5	g	Riboflavin	
0.5	g	Folic acid	
1000	ml	Distilled water	


## S I

6	g	K2HPO4	
1000	ml	Distilled water	


## S II

6	g	KH2PO4	
12	g	(NH4)2SO4	
12	g	NaCl	
1.2	g	MgSO4 x 7 H2O	
1.2	g	CaCl2 x 2 H2O	
1000	ml	Distilled water