# 1416: MST-MEDIUM, AUTOTROPHIC GROWTH


## Main sol. 1416

5	g	Na2S2O3 x 5 H2O	
1	g	NH4Cl	
0.5	g	MgSO4 x 7 H2O	
4	g	K2HPO4	
1.5	g	KH2PO4	
1	ml	**Trace element solution SL-10**	
1000	ml	Distilled water	

1. Prepare separate solutions of sodium thiosulfate and magnesium sulfate. Adjust solutions to pH 7.5 -8.0 and sterilize by autoclaving. Add sterile trace element solution after autoclving and combining the solutions.


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.