# 1425: SSE/HD 1:10 MEDIUM (HEPES)


## Main sol. 1425

0.5	g	Peptone	
0.25	g	Yeast extract	
0.1	g	Glucose	
1.95	g	HEPES (10 mM final concentration)	
500	ml	**SSE (double concentrated)**	
1	ml	**Trace element solution SL-10**	
500	ml	Distilled water	

1. Add to 1000 ml of medium after autoclaving: 

1	ml	**Vitamin solution**	

2. adjust pH to 7.0


## SSE (double concentrated)

0.2938	g	CaCl2 x 2 H2O	
0.1069	g	NH4Cl	
0.2036	g	MgCl2 x 6 H2O	
0.1983	g	(NH4)2SO4	
0.739	g	MgSO4 x 7 H2O	
0.8606	g	CaSO4 x 2 H2O	
0.236	g	Ca(NO3)2 x 4 H2O	
0.424	g	Na NO3	
0.07	g	KH2PO4	
0.0111	g	FeSO4 x 7 H2O	
0.087	g	K2SO4	
1000	ml	Distilled water	

1. Dissolve by shaking overnight. Sterilize by autoclaving.


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.


## Vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine-HCl x 2 H2O	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	D-Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water