# 1431: ALKALOPHILIC HALOPHILE MEDIUM


## Main sol. 1431

10	g	Yeast extract	
7.5	g	Casamino acids	
3	g	Trisodium citrate	
2	g	KCl	
1	g	MgSO4 x 7 H2O	
0.36	mg	MnCl2 x 4 H2O	
50	mg	FeSO4 x 7 H2O	
200	g	NaCl	
50	g	Na2CO3	
2	%	Agar	

1. Liquid medium may be prepared by adding all components together. Agar (2%) medium should be prepared without Na2CO3 and sterilised by autoclaving. Add 20-30 ml of a sterile 10% Na2CO3 solution to the autoclaved medium containing agar so that the final pH is 9.0.