# 1454: Meiothermus hypogaeus MEDIUM


## Main sol. 1454

1	g	Yeast extract (Difco, Bacto )	
1	g	Bacto Tryptone (Difco)	
1	g	Sodium glutamate monohydrate	
0.06	g	CaSO4 x 2 H2O	
0.1	g	MgSO4 x 7 H2O	
8	mg	NaCl	
0.1	g	KNO3	
0.69	g	NaNO3	
0.11	g	Na2HPO4	
10	ml	**Trace element solution**	
20	g	Agar	
1000	ml	Distilled water	

1. Adjust pH to 7.5 with NaOH.


## Trace element solution

12.8	g	Nitrilotriacetic acid (NTA)	
1.35	g	FeCl3 x 6 H2O	
0.1	g	MnCl2 x 4 H2O	
24	mg	CoCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
0.1	g	ZnCl2	
25	mg	CuCl2 x 2 H2O	
0.01	g	H3BO3	
24	mg	Na2MoO4 x 2 H2O	
1	g	NaCl	
0.12	g	NiCl2 x 6 H2O	
4	mg	Na2SeO4	
4	mg	Na2WO4	
1000	ml	Distilled water	

1. First dissolve the nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.