# 1461: SALINIVIBRIO SHARMENSIS MEDIUM


## Main sol. 1461

100	g	NaCl	
3	g	Trisodium citrate	
1	g	MgSO4 x 7 H2O	
2	g	KCl	
3	g	Na2CO3	
1	ml	MnCl2 x 4 H2O (0.36 g/l)	
1	ml	FeSO4 x 7 H2O (50 g/l)	
10	g	Yeast extract (Difco)	
1000	ml	Distilled water	
20	g	Agar, for solid medium	

1. Final pH 9.0
2. Add all the components together, except Na2CO3 which is added from a sterilized stock solution when the autoclaved medium has cooled. Agar is added at a concentration of 20 g/l for solid media and should be cooled to about 55°C before the Na2CO3.is added.