# 1473: PLANTOMYCETACEAE MEDIUM ## Main sol. 1473 0.33 g KH2PO4 0.33 g NH4Cl 0.33 g MgCl2 0.33 g CaCl2 0.33 g KCl 1 ml **Trace Element Solution** 1000 ml Distilled water 1. Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions: 1 g KNO3 2 g Glucose 0.05 g Yeast extract (Sigma) 1 ml **Wolin's vitamin solution** ## Trace Element Solution 784 mg Fe(NH4)2(SO4)2 x 6 H2O 284 mg NiCl2 x 2 H2O 38 mg CoCl2 x 6 H2O 99 mg MnCl2 x 4 H2O 144 mg ZnSO4 x 7 H2O 95 mg Na2SeO4 33 mg Na2WO4 x 2 H2O 24 mg Na2MoO4 x 2 H2O 6 mg H3BO3 2 mg CuCl2 x 2 H2O 1000 ml Distilled water ## Wolin's vitamin solution 2 mg Biotin 2 mg Folic acid 10 mg Pyridoxine hydrochloride 5 mg Thiamine HCl 5 mg Riboflavin 5 mg Nicotinic acid 5 mg Calcium D-(+)-pantothenate 0.1 mg Vitamin B12 5 mg p-Aminobenzoic acid 5 mg (DL)-alpha-Lipoic acid 1000 ml Distilled water