# 1485: DESULFONATRONUM ALKALITOLERANS MEDIUM


## Main sol. 1485

950	ml	**Solution A**	
1	ml	**Solution B**	
1	ml	**Solution C**	
10	ml	**Solution D**	
10	ml	**Solution E**	
10	ml	**Solution F**	
10	ml	**Solution G**	
10	ml	**Solution H**	

1. Dissolve ingredients of solution A except carbonates, sparge with 100% N2 gas for 30 - 45 min to make it anoxic, then add bicarbonate and carbonate, adjust pH to 9.0, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of sterile solutions B to H prepared under 100% N2 gas atmosphere. Adjust pH of the complete medium to 8.7 - 9.0, if necessary.


## Solution A

6	g	NaCl	
1	g	K2HPO4	
32	g	NaHCO3	
10.6	g	Na2CO3	
2.8	g	Na2SO4	
0.2	g	Na-acetate	
950	ml	Distilled water	


## Solution B

1	ml	**Trace elements solution (Pfennig & Lippert,1966)**	


## Solution C

1	ml	**Selenite-tungstate solution**	


## Solution D

0.2	g	NH4Cl	
10	ml	Distilled water	


## Solution E

0.2	g	MgCl2 x 6 H2O	
10	ml	Distilled water	


## Solution F

20	mg	Yeast extract	
10	ml	Distilled water	


## Solution G

3.4	g	Na-formate	
10	ml	Distilled water	


## Solution H

0.3	g	Na2S x 9 H2O	
10	ml	Distilled water	


## Trace elements solution (Pfennig & Lippert,1966)

5	g	EDTA	
2.2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.03	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.03	g	CuCl2 x 2 H2O	
0.03	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. pH 3.0-4.0


## Selenite-tungstate solution

0.5	g	NaOH	
3	mg	Na2SeO3 x 5 H2O	
4	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water