# 1497: HALOARCHAEUM SALINUM MEDIUM


## Main sol. 1497

500	ml	**Mineral Salts Solution for solid Medium**, alternative	
500	ml	**Mineral Salts Solution for liquid Medium**, alternative	


## Mineral Salts Solution for solid Medium

1	g	L-Glutamic acid	
1	g	Trisodium citrate x 2 H2O	
0.5	g	KH2PO4	
0.5	g	NH4Cl	
2.5	g	K2SO4	
20	g	MgCl2 x 7 H2O	
2	g	Casamino acids	
1	g	Yeast extract (Oxoid)	
2	mg	FeSO4	
1	ml	**Trace Element Solution**	
300	ml	Distilled water	
100	g	NaCl	
20	g	Agar	
200	ml	Distilled water	


## Mineral Salts Solution for liquid Medium

1	g	L-Glutamic acid	
1	g	Trisodium citrate x 2 H2O	
0.5	g	KH2PO4	
0.5	g	NH4Cl	
2.5	g	K2SO4	
20	g	MgCl2 x 7 H2O	
100	g	NaCl	
2	g	Casamino acids	
1	g	Yeast extract	
2	mg	FeSO4	
1	ml	**Trace Element Solution**	
500	ml	Distilled water	

1. Adjust to pH 5.5 with 10% KOH and sterilize by autoclaving.


## Trace Element Solution

0.3	g	CoCl2 x 6 H2O	
0.1	g	MnCl2 x 4 H2O	
0.1	g	ZnCl2	
0.02	g	H3BO4	
0.1	g	Na2MoO4 x 2 H2O	
0.1	g	NiCl2 x 6 H2O	
0.01	g	CuCl2 x 6 H2O	
0.04	g	AlCl3	
0.02	g	Na2WO4 x 2 H2O	
1	g	CaCl2 x 2 H2O	
0.01	g	BaCl2 x 2 H2O	
2	g	Na2S2O3 x 5 H2O	
1000	ml	Distilled water	

1. Adjust to pH 4.0.