# 1521: ARCOBACTER ANAEROPHILUS MEDIUM


## Main sol. 1521

0.5	g	KH2PO4	
20	g	NaCl	
0.25	g	CaCl2 x 2 H2O	
1	g	MgSO4 x 7 H2O	
0.5	g	NH4Cl	
0.3	g	Sodium citrate	
0.5	g	Yeast extract	
1	g	Ammonium acetate	
1	g	Lactate	
1	g	Pyruvate	
1	ml	SL10	
1	mg	Resazurin	
1000	ml	Distilled water	

1. Add distilled water to 1 liter, boil the medium and cool under N2, distribute under N2 and autoclave. After autoclaving the following sterilized solutions are added: 

10	ml	NaHCO3 (10%)	
10	ml	Na2S x 9 H2O (1 M)	
10	ml	Vitamin B12