# 1524: PLANCTOMYCETES MEDIUM NH11 ## Main sol. 1524 20 ml **Mineral salt solution** 0.5 g Peptone (Bacto) 0.25 g Glucose 2.38 g HEPES (10mM) 500 ml **Artifical sea water** 15 g Agar (Bacto) 475 ml Distilled water 1. Sterilize 20 min/121°C. After cooling to 60°C add to the medium: 5 ml **Vitamin solution (double conc.)** 2. Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH). ## Mineral salt solution 10 g Nitrilotriacetic acid (NTA) 29.7 g MgSO4 x 7 H2O 3.34 g CaCl2 x 2 H2O 12.67 mg Na2MoO4 x 2 H2O 99 mg FeSO4 x 7 H2O 50 ml Metall salt sol. 44 (see medium 590) 900 ml Distilled water 1. Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water. ## Vitamin solution (double conc.) 4 mg Biotin 4 mg Folic acid 20 mg Pyridoxine hydrochloride 10 mg Riboflavine 10 mg Thiamine-HCl x 2 H2O 10 mg Nicotinamide 10 mg D-Calcium pantothenate 0.2 mg Vitamin B12 10 mg p-Aminobenzoic acid 1000 ml Distilled water 1. Store in the dark and cold (5°C). ## Artifical sea water 23.47 g NaCl 3.92 g Na2SO4 4.98 g MgCl2 x 6 H2O 1.1 g CaCl2 192 mg NaHCO3 664 mg KCl 96 mg KBr 26 mg H3BO3 24 mg SrCl2 3 mg NaF 1000 ml Distilled water