# 153: THIOGLYCOLATE MEDIUM (MERCK 108190)


## Main sol. 153

15	g	Trypticase (BBL)	
0.5	g	L-Cystine	
2	g	Glucose	
5	g	Yeast extract	
2.5	g	NaCl	
0.5	g	Na-thioglycolate	
0.5	ml	Sodium resazurin (0.1% w/v)	
15	g	Agar, for solid medium (optional)	
1000	ml	Distilled water	

1. Dissolve ingredients, sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.1 with Na2CO3 (5% w/v), if necessary. Then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.