# 1536: BAP+ MEDIUM FOR FRANKIA ## Main sol. 1536 0.95 g KH2PO4 0.6 g K2HPO4 0.27 g NH4Cl 0.48 g Na-propionate 0.025 g MgSO4 x 7 H2O 0.01 g CaCl2 x 2 H2O 1 ml **Vitamine solution** 1 ml **Chelated iron solution** 0.1 ml **Trace element solution** 1000 ml Distilled water 1. The pH of the final solution is adjusted to 6.3. 2. Note: Please autoclave the chelated iron solution before you add to the medium, so that solves everything!!!! ## Vitamine solution 0.01 g Thiamine HCl 0.05 g Nicotinic acid 0.05 g Pyridoxine hydrochloride 100 ml Distilled water ## Chelated iron solution 1 g Citric acid 1 g Eisencitrat 100 ml Distilled water ## Trace element solution 1 mg CoSO4 x 7 H2O 0.08 g CuSO4 x 5 H2O 2.86 g H3BO3 1.81 g MnCl2 x 4 H2O 0.025 g Na2MoO4 x 2 H2O 0.22 g ZnSO4 x 7 H2O 100 ml Distilled water