# 1578: 1/10 R2A MEDIUM


## Main sol. 1578

0.05	g	Yeast extract	
0.05	g	Proteose peptone (Difco no. 3)	
0.05	g	Casamino acids	
0.05	g	Glucose	
0.05	g	Starch (soluble)	
0.03	g	Na-pyruvate	
0.03	g	K2HPO4	
5	mg	MgSO4 x 7 H2O	
15	g	Agar	
1000	ml	Distilled water	

1. Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.