# 158: THERMOPLASMA ACIDOPHILUM MEDIUM


## Main sol. 158

1.32	g	(NH4)2SO4	
0.372	g	KH2PO4	
0.247	g	MgSO4 x 7 H2O	
0.074	g	CaCl2 x 2 H2O	
10	ml	**Trace element solution**	
2	g	Yeast extract (Oxoid)	
20	g	Glucose	
1000	ml	Distilled water (freshly distilled)	

1. Adjust pH to 1.0 with 1 N H2SO4. Stock solutions of yeast extract (10% w/v) and glucose (50% w/v) in water are sterilized separately by autoclaving and thereafter added to the sterile mineral salt medium.


## Trace element solution

1.93	g	FeCl3 x 6 H2O	
0.18	g	MnCl2 x 4 H2O	
0.45	g	Na2B4O7 x 10 H2O	
22	mg	ZnSO4 x 7 H2O	
5	mg	CuCl2 x 2 H2O	
3	mg	Na2MoO4 x 2 H2O	
3.8	mg	VOSO4 x 5 H2O	
2	mg	CoSO4 x 7 H2O	
1000	ml	Distilled water