# 1586: ISP2 MODIFIED MEDIUM FOR METHYLIBIUM


## Main sol. 1586

4	g	Yeast extract (Difco)	
10	g	Malt extract (Difco)	
4	g	Dextrose (Difco)	
1	ml	**Trace element solution SL-10**	
20	g	Agar	
1000	ml	Distilled water	

1. pH 7.2
2. Sterilize 20 min/121°C. After cooling to 60°C add to the medium: 

5	ml	**Vitamin solution**	


## Trace element solution SL-10

10	ml	HCl (25%; 7.7 M)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	


## Vitamin solution

0.2	mg	Vitamin B12	
4	mg	Biotin	
10	mg	Thiamine-HCl x 2 H2O	
10	mg	Calcium pantothenate	
1	mg	Folic acid	
10	mg	Riboflavin	
10	mg	Nicotinamide	
10	mg	p-Aminobenzoic acid	
10	mg	Pyridoxine hydrochloride	
1000	ml	Distilled water	

1. Filter-sterilized.