# 1606: RUBROBACTER CARBOXYDOVORANS MEDIUM


## Main sol. 1606

4.5	g	Na2HPO4 x 2 H2O	
1.5	g	KH2PO4	
0.01	g	MnSO4 x H2O	
0.2	g	MgSO4 x 7 H2O	
0.01	g	CaCl2 x 2 H2O	
5	mg	Ferric ammonium citrate	
3	ml	**Trace element solution SL-6**	
1	g	Yeast extract	
1	g	Tryptone	
1000	ml	Distilled water	
15	g/l	Agar, for solid medium	

1. Adjust pH to 8.0. Agar if necessary 15.0 g/l. Incubate without agitation at 50°C.


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water