# 162: HYPHOMICROBIUM MEDIUM


## Main sol. 162

1.36	g	KH2PO4	
2.13	g	Na2HPO4	
0.5	g	(NH4)2SO4	
0.2	g	MgSO4 x 7 H2O	
6	mg	CaCl2 x 2 H2O	
3	mg	FeSO4 x 7 H2O	
1	mg	MnSO4 x H2O	
1.5	mg	Na2MoO4 x 2 H2O	
3.38	g	Methylamine hydrochloride	
0.1	g	Yeast extract	
1000	ml	Distilled water	

1. Adjust pH to 7.4 with NaOH. Autoclave 20 min at 121°C; final pH: 7.2.