# 1629: MINIMAL MEDIA CONTAINING METHANOL ## Main sol. 1629 1.62 g NH4Cl 0.2 g MgSO4 x 7 H2O 2.4 g K2HPO4 1.1 g NaH2PO4 x 2 H2O 15 mg Na2-EDTA 3 mg FeSO4 x 6 H2O 4.5 mg ZnSO4 x 7 H2O 3 mg CoCl2 x 6 H2O 0.64 mg MnCl2 x 4 H2O 1 mg H3BO3 0.4 mg Na2MoO4 x 2 H2O 0.3 mg CuSO4 x 2 H2O 3 mg CaCl2 x 6 H2O 5 ml Methanol 15 g Agar 1000 ml Distilled water 1. Prepare the medium without methanol. Adjust pH to 7.1. 2. Autoclave and after cooling to 50°C add the methanol from a filter-sterilized stock solution.