# 1631: VERRUCOMICROBIUM MEDIUM (IG) ## Main sol. 1631 20 ml **Mineral salt solution** (medium 621) 0.25 g Peptone (Bacto) 0.25 g Yeast extract (Bacto) 2.38 g HEPES 15 g Agar (Bacto) 965 ml Distilled water 1. Sterilize 20 min./121°C. After cooling to 60°C add to the medium: 10 ml Glucose (2.5%, sterile-filtered) 5 ml **Vitamin solution (double conc.)** 2. Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH). ## Mineral salt solution 10 g Nitrilotriacetic acid (NTA) 29.7 g MgSO4 x 7 H2O 3.34 g CaCl2 x 2 H2O 12.67 mg Na2MoO4 x 2 H2O 99 mg FeSO4 x 7 H2O 50 ml Metall salt sol. 44 (see medium 590) 900 ml Distilled water 1. Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water. ## Vitamin solution (double conc.) 4 mg Biotin 4 mg Folic acid 20 mg Pyridoxine hydrochloride 10 mg Riboflavine 10 mg Thiamine-HCl x 2 H2O 10 mg Nicotinamide 10 mg D-Calcium pantothenate 0.2 mg Vitamin B12 10 mg p-Aminobenzoic acid 1000 ml Distilled water 1. Store in the dark and cold (5°C).