# 1639: AZOARCUS TAIWANENSIS MEDIUM ## Main sol. 1639 10 g NaCl 0.76 g KNO3 0.1 g NH4Cl 0.25 g K2HPO4 1 ml **Trace element solution SL-11** 1.5 g NaHCO3 0.44 g Na-acetate 0.1 g Na2S x 9 H2O 1000 ml Distilled water 1. Dissolve ingredients (except bicarbonate, acetate and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then, add and dissolve bicarbonate, adjust pH to 8.5 - 9.0, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add acetate and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Adjust pH of complete medium to 8.5 - 9.0, if necessary. ## Trace element solution SL-11 5.2 g Na2-EDTA x 2 H2O 1.5 g FeCl2 x 4 H2O 70 mg ZnCl2 100 mg MnCl2 x 4 H2O 6 mg H3BO3 190 mg CoCl2 x 6 H2O 2 mg CuCl2 x 2 H2O 24 mg NiCl2 x 6 H2O 36 mg Na2MoO4 x 2 H2O 1000 ml Distilled water 1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.