# 1647: ACETOBACTEROIDES MEDIUM


## Main sol. 1647

0.2	g	Yeast extract	
0.18	g	Glucose	
0.14	g	KH2PO4	
0.2	g	MgCl2 x 6 H2O	
0.15	g	CaCl2 x 2 H2O	
0.54	ml	NH4Cl	
0.3	g	L-Cysteine HCl x H2O	
2.5	g	NaHCO3	
0.3	g	Na2S x 9 H2O	
1	mg	Resazurin	
1	ml	**Vitamin solution**	
1	ml	**Trace element solution**	
1	ml	**Se/W solution**	
1000	ml	Distilled water	

1. The vitamin solution, trace element solution, Se/W solution, cysteine, glucose, Na2S and NaHCO3 are added after the medium has been autoclaved.


## Se/W solution

4	mg	Na2SeO3	
4	mg	Na2WO4	
1000	ml	Distilled water	


## Vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine-HCl x 2 H2O	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	D-Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water	

1. Note: for preparation see Medium 141


## Trace element solution

12.8	g	Nitrilotriacetic acid (NTA)	
1	g	FeCl2 x 4 H2O	
0.1	g	MnCl2 x 4 H2O	
0.03	g	CoCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
0.1	g	ZnCl2	
0.02	g	CuCl2	
0.01	g	H3BO3	
0.03	g	Na2MoO4 x 2 H2O	
0.1	g	NiCl2 x 6 H2O	
1	g	NaCl	
0.03	g	Na2SeO3 x 5 H2O	
0.04	g	Na2WO4 x 2 H2O	
1000	ml	Distilled water	

1. First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.