# 1655: MODIFIED THERMUS MEDIUM 878


## Main sol. 1655

2.5	g	Yeast extract	
2.5	g	Tryptone	
28	g	Agar	
100	mg	Nitrilotriacetic acid	
40	mg	CaSO4 x 2 H2O	
200	mg	MgCl2 x 6 H2O	
0.5	ml	Fe(III) citrate (0.01 M)	
0.5	ml	**Trace element solution**	
100	ml	**Phosphate buffer**	
900	ml	Distilled water	

1. Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min.
2. Autoclave the phosphate buffer separately and then add to the medium.


## Phosphate buffer

5.44	g	KH2PO4	
43	g	Na2HPO4 x 12 H2O	
1000	ml	Distilled water	

1. Adjust pH to 7.2


## Trace element solution

0.5	ml	H2SO4	
2.28	g	MnSO4 x H2O	
0.5	g	ZnSO4 x 7 H2O	
0.5	g	H3BO3	
25	mg	CuSO4 x 5 H2O	
25	mg	Na2MoO4 x 2 H2O	
45	mg	CoCl2 x 6 H2O	
1000	ml	Distilled water