# 166: HYPHOMICROBIUM STRAIN X MEDIUM


## Main sol. 166

1	g	(NH4)2SO4	
0.2	g	MgSO4 x 7 H2O	
0.5	g	NaH2PO4 x H2O	
1.55	g	K2HPO4	
0.2	ml	**Trace element solution (Vishniac & Santer, 1957)**	
3.4	g	Methylamine hydrochloride	
15	g	Agar	
1000	ml	Distilled water	

1. Adjust pH to 7.2. For liquid medium use only 0.7 g of methylamine hydrochloride per liter.


## Trace element solution (Vishniac & Santer, 1957)

50	g	Na2-EDTA	
22	g	ZnSO4 x 7 H2O	
5.54	g	CaCl2 x 2 H2O	
5.06	g	MnCl2 x 4 H2O	
5	g	FeSO4 x 7 H2O	
1.1	g	(NH4)6Mo7O24 x 4 H2O	
1.57	g	CuSO4 x 5 H2O	
1.61	g	CoCl2 x 6 H2O	
1000	ml	Distilled water	

1. Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.