# 1660: modified BAP+ (PSA) MEDIUM FOR FRANKIA


## Main sol. 1660

0.95	g	KH2PO4	
0.6	g	K2HPO4	
0.27	g	NH4Cl	
0.48	g	Na-propionate	
0.48	g	Sodium succinate dibasic	
0.48	g	Pyruvic acid sodium salt	
0.48	g	Sodium acetate	
0.025	g	MgSO4 x 7 H2O	
0.01	g	CaCl2 x 2 H2O	
1	ml	**Vitamine solution**	
1	ml	**Chelated iron solution**	
0.1	ml	**Trace element solution**	
1000	ml	Distilled water	

1. The pH of the final solution is adjusted to 6.3
2. Note: Please autoclave the chelated iron solution before you add to the medium, so that
solves everything!!!!


## Vitamine solution

0.01	g	Thiamine HCl	
0.05	g	Nicotinic acid	
0.05	g	Pyridoxine hydrochloride	
100	ml	Distilled water	


## Chelated iron solution

1	g	Citric acid	
1	g	Eisencitrat	
100	ml	Distilled water	


## Trace element solution

1	mg	CoSO4 x 7 H2O	
0.08	g	CuSO4 x 5 H2O	
2.86	g	H3BO3	
1.81	g	MnCl2 x 4 H2O	
0.025	g	Na2MoO4 x 2 H2O	
0.22	g	ZnSO4 x 7 H2O	
100	ml	Distilled water