# 1664: Sphaerotilus natans subspec. sulfidivorans medium


## Main sol. 1664

0.3	g	(NH4)Cl	
9	mg	KH2PO4	
22	mg	K2HPO4	
35	mg	Na2HPO4 x 7 H2O	
23	mg	MgSO4 x 7 H2O	
28	mg	CaCl2	
0.25	mg	FeCl3 x 6 H2O	
200	mg	Na-lactate	
500	mg	Peptone	
1	g	Na2S2SO3	
25	μl	**Trace element solution**	
1000	ml	Distilled water	
25	μl	**Standard vitamin solution**	

1. Dissolve ingredients (except vitamins) in distilled water.
2. After sterilization add the 25 µl vitamin solution.


## Trace element solution

0.2	g	EDTA	
0.7	g	FeSO4 x 7 H2O	
0.01	g	ZnSO4 x 7 H2O	
2	mg	MnSO4 x 4 H2O	
5	mg	CuSO4 x 5 H2O	
0.01	g	H3BO3	
1	g	Co(NO3)2	
1	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## Standard vitamin solution

10	mg	Riboflavin	
50	mg	Thiamine-HCl x 2 H2O	
50	mg	Nicotinic acid	
50	mg	Pyridoxine hydrochloride	
50	mg	Calcium pantothenate	
0.1	mg	Biotin	
0.2	mg	Folic acid	
1	mg	Vitamin B12	
100	ml	Distilled water