# 1668: MDM (modified dehority medium)


## Main sol. 1668

0.5	g	Yeast extract	
0.5	g	Tryptone	
250	ml	**Stock A: Salt solution 2x (50 ml stock per 100 ml medium)**	
2.5	ml	**Stock B: Salt solution 200x (0,5 ml stock per 100 ml medium)**	
250	ml	Distilled water	
1	g/l	L-Cysteine HCl	

1. To make medium anoxic boil it, cool under N2CO2 gas atmosphere, add 1 g/l L-cysteine hydrochloride and adjust pH to 6.8. Finally gas over with N2 . Dispense under N2CO2 into suitable vessels for anaerobic growth (serum bottles or Hungate tubes sealed with rubber stoppers) and sterilize by autoclaving.


## Vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine-HCl x 2 H2O	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	D-Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water	

1. Filter sterilize and make anoxic with N2.


## Cellobiose (10%)

100	ml	Distilled water	
10	g	Cellobiose	

1. Mix 10 g cellobiose with 100 ml distilled water and filter sterilize, make anoxic with N2.


## CM-Mix (only for DSM 104697)

4	g	D-Glucose	
1	g	Cellobiose	
1	g	Maltose	
1	g	Starch (soluble)	
1000	ml	Distilled water	

1. Make anoxic with N2.


## Vitamin K1 solution (only for DSM 104697)

20	ml	Ethanol (95 %)	
0.1	ml	Vitamin K1	

1. Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Make anoxic with N2. Store refrigerated in a brown bottle.


## Stock A: Salt solution 2x (50 ml stock per 100 ml medium)

6804	mg	K2HPO4	
6721	mg	NaHCO3	
4675	mg	NaCl	
1605	mg	NH4Cl	
89	mg	CaCl2	
95	mg	MgCl2	
28	mg	FeSO4 x 7 H2O	
1000	ml	Distilled water	


## Stock B: Salt solution 200x (0,5 ml stock per 100 ml medium)

971	mg	MgCl2 x 2 H2O	
273	mg	ZnCl2	
104	mg	CoCl2	
48	mg	Na2 MoO4 x 2 H2O	
35	mg	Na2SeO3	
237	mg	NiCl2 x 6 H2O	
66	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water