# 1679: Cyanobacteria Medium 2xASN3


## Main sol. 1679

200	g/l	NaCl (250 g/l stock solution)	
20	g/l	MgCl2 x 6 H2O (200 g/l stock solution)	
20	g/l	KCl (50 g/l stock solution)	
20	g/l	Mg(SO4) x 7 H2O (350 g/l stock solution)	
20	g/l	CaCl2 x 2 H2O (50 g/l stock solution)	
10	ml/l	Na3-citrate x 2 H2O (0.6 g/l stock solution)	
10	ml/l	Na2-EDTA x 2 H2O (0.1 g/l stock solution)	
1	ml	**T-Trace Metal Mix**	
1000	ml	Distilled water	

1. Adjust to 1000 mL with H2O dist. and autoclave.
2. After cooling, add the following filter sterilized components:

2.5	ml/l	NaNO3 (300 g/l stock solution)	
2.5	ml/l	K2HPO4 x 3 H2O (8.0 g/l stock solution)	
1	ml/l	Na2CO3 (20 g/l stock solution)	
0.5	ml/l	Fe(NH4)citrate (6.0 g/l stock solution)	
1	ml/l	Vitamin B12 (0.02 g/l stock solution)	


## T-Trace Metal Mix

2.9	g/l	H3BO3	
1.81	g/l	MnCl2 x 4 H2O	
0.22	g/l	ZnSO4 x 7 H2O	
0.39	g/l	Na2MoO4 x 2 H2O	
10	ml/l	CuSO4 x 5 H2O (0.08 g/l stock solution)	
10	ml/l	Co(NO3)2 x 6 H2O (0.05 g/l stock solution)