# 1680: Cyanobacteria Medium MCL


## Main sol. 1680

33	g	Artificial Sea Salt (hw Marinemix 21010; Wiegandt GmbH – Products for aquaristics, Sterkenhofweg 13, 47807 Krefeld, Germany)	
5	ml	**MCL Stock Solution A ***	
5	ml	**MCL Stock Solution B ****	

1. Adjust to 1000 ml with MilliQ water and autoclave (121°C, 20 min).
2. After cooling add the following filter sterilized component under sterile conditions:

1	ml	**MCL Vitamin Stock Solution *****	


## MCL Stock Solution A *

5	ml	CuSO4 x 7 H2O (1.92 g/l stock solution)	
100	ml	MnSO4 x H2O (2.4 g/l stock solution)	
100	ml	ZnSO4 x 7 H2O (0.44 g/l stock solution)	
100	ml	Fe(NH4)2(SO4)2 x 6 H2O (5.6 g/l stock solution)	

1. Adjust to 500 ml with MilliQ water.


## MCL Stock Solution B **

100	ml	FeCl3 (0.96 g/l stock solution)	
100	ml	H3BO3 (22.4 g/l stock solution)	
100	ml	NaNO3 (84.0 g/l stock solution)	
100	ml	Na2-EDTA x 2 H2O (25.3 g/l stock solution)	
100	ml	Sodium beta-glycerophosphate (8.0 g/l stock solution)	

1. Mix to obtain 500 ml of MCL Stock Solution B.


## MCL Vitamin Stock Solution ***

160	μl	Vitamin B12 (1.0 mg/ml stock solution)	
160	μl	Biotin (1.0 mg/ml stock solution)	
800	μl	Thiamine (10.0 mg/ml stock solution)	

1. Adjust to 100 ml with MilliQ water.