# 1713: GAM Broth (Gifu Anaerobic Medium Broth) ## Main sol. 1713 59 g GAM Broth powder (HyServe) 1000 ml Deionized water 1. Suspend the dehydrated medium in deionized water, and heat do dissolve the medium, sterilize by autoclaving at 115 °C for 15 minutes.