# 172: CYTOPHAGA (marine) MEDIUM


## Main sol. 172

1	g	Yeast extract (Difco)	
1	g	Tryptone (Difco)	
24.7	g	NaCl	
0.7	g	KCl	
6.3	g	MgSO4 x 7 H2O	
4.6	g	MgCl2 x 6 H2O	
1.2	g	CaCl2 x 2 H2O	
0.2	g	NaHCO3	
15	g	Agar (Difco)	
1000	ml	Distilled water	

1. Adjust pH to 7.2. Sodium bicarbonate and calcium chloride are autoclaved separately, each in a small volume of distilled water.