# 1721: MMJHS MEDIUM


## Main sol. 1721

1	g	NaNO3	
1	g	NaHCO3	
1	g	Na2S2O3 x 5 H2O	
10	g	Sulfur (powdered)	
1000	ml	** Synthetic seawater** (MJ)	

1. Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding NaNO3, Na2S2O3 x 5 H20 and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, NaNO3, Na2S2O3 x 5 H20 and NaHCO3.
2. Increase the 80% H2 + 20% CO2 gas phase pressure to 0.3 MPa. The final pH is 7.0.


##  Synthetic seawater

25	g	NaCl	
0.14	g	K2HPO4	
0.7	g	CaCl2 x 2 H2O	
0.25	g	NH4Cl	
3.4	g	MgSO4 x 7 H2O	
4.2	g	MgCl2 x 6 H2O	
0.5	g	KCl	
10	ml	**Trace mineral solution**	
1000	ml	Distilled water	


## Trace mineral solution

1.5	g	Nitrilotriacetic acid	
3	g	MgSO4 x 7 H2O	
0.5	g	MnSO4 x n H2O	
1	g	NaCl	
0.1	g	FeSO4 x 7 H2O	
0.18	g	CoSO4 x 7 H2O	
0.1	g	CaCl2 x 2 H2O	
0.18	g	ZnSO4 x 7 H2O	
0.01	g	CuSO4 x 5 H2O	
2	mg	AlK(SO4)2	
0.01	g	H3BO3	
0.1	g	Na2WO4 x 2 H2O	
0.025	g	NiCl2 x 6 H2O	
1000	ml	Distilled water	
0.01	g	Na2MoO4 x 2 H2O	
0.05	g	Na2SeO3 x 5 H2O	

1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).