# 1727: Z45/4 Medium for Cyanobacteria


## Main sol. 1727

41	mg	KH2PO4	
17	mg	K2HPO4	
37	mg	CaCl2 x 2 H2O	
25	mg	MgSO4 x 7 H2O	
10	ml	** Fe-EDTA complex**	
0.06	ml	**Micronutrient solution (after H. Gaffron)**	
1000	ml	Distilled water (or deionized water)	


##  Fe-EDTA complex

1. Combine in deionized or distilled water and make up to 500 ml: 

135	mg	FeCl3 x 6 H2O	
186	mg	Na-EDTA	


## Micronutrient solution (after H. Gaffron)

1. Add to 100 ml of deionized or distilled water: 

310	mg	H3BO3	
223	mg	MnSO4 x H2O	
3.3	mg	Na2WO4 x 2 H2O	
8.8	mg	(NH4)6Mo7O24 x 4 H2O	
11.9	mg	KBr	
8.3	mg	KJ	
28.7	mg	ZnSO4 x 7 H2O	
15.4	mg	Cd(NO3)2 x 4 H2O	
14.6	mg	Co(NO3)2 x 6 H2O	
12.5	mg	CuSO4 x 5 H2O	
19.8	mg	(NH4)2Ni(SO4)2 x 6 H2O	
3.7	mg	Cr(NO3)3 x 7 H2O	
2	mg	VOSO4 x 2 H2O	
47.4	mg	AlK(SO4)2 x 12 H2O