# 1790: Mucispirillum Spezial


## Main sol. 1790

9.26	g	Brain heart infusion	
7.5	g	Trypticase soy broth	
2.5	g	Yeast extract	
0.426	g	NaNO3	
1.25	g	K2HPO4	

1. Adjust pH to 7.0.
2. Bring to boil, cool down under N2CO2.

0.25	g	Cysteine HCl	

3. Fill under N2CO2 in hungate tubes (10 ml)
4. Autoclave at 121 °C for 15 min.

0.05	ml	**Haemin solution**	
0.05	ml	**Vitamin K3 solution**	
0.5	ml	Fetal bovine serum	

5. pressurize with 1 bar H2


## Haemin solution

50	mg	Haemin	
1	ml	NaOH (1 N)	
100	ml	Distilled water	

1. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.


## Vitamin K3 solution

5	mg	Vitamin K3	
1	ml	Ethanol (95%)	
100	ml	Water	

1. Dissolve 5 mg vitamin K3 in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle.