# 181: SELENOMONAS RUMINANTIUM MEDIUM ## Main sol. 181 1 g Glucose 5 g Trypticase (BBL) 1 g KH2PO4 4 g Na-acetate 2 g Yeast extract 0.1 ml n-Valeric acid 1 mg Resazurin 4 g Na2CO3 0.5 g L-Cysteine HCl x H2O 1000 ml Distilled water 1. Adjust pH to 7.0. Gas atmosphere: 100% CO2. 2. Distribute into Hungate tubes and autoclave.