# 182: SACCHAROLOBUS SOLFATARICUS MEDIUM


## Main sol. 182

1	g	Yeast extract (OXOID)	
1	g	Casamino acids (BD BBL)	
3.1	g	KH2PO4	
2.5	g	(NH4)2SO4	
0.2	g	MgSO4 x 7 H2O	
0.25	g	CaCl2 x 2 H2O	
10	ml	**Allen's trace element solution**	
1000	ml	Distilled water	

1. Dissolve all compounds except yeast extract and Casamino acids, adjust pH to 4.0 - 4.2 with 10 N H2SO4 at room temperature and autoclave. Prior to inoculation add yeast extract and Casamino acids from stock solutions sterilized by filtration.


## Allen's trace element solution

180	mg	MnCl2 x 4 H2O	
450	mg	Na2B4O7 x 10 H2O	
22	mg	ZnSO4 x 7 H2O	
5	mg	CuCl2 x 2 H2O	
3	mg	Na2MoO4 x 2 H2O	
3	mg	VOSO4 x 2 H2O	
1	mg	CoSO4 x 7 H2O	
1000	ml	Distilled water	

1. Adjust pH of final solution to 2 with 1 N HCl.